Article Details

Background:

Chick embryos are mostly used as developmental model system to study the potential effects of various drugs and their mechanism of action. However inaccessibility of developing chick embryo in the egg has led to partial understanding of the effects and mechanisms of various drugs. Hence, an experiment to design and develop a culture vessel and culture medium to support growth of chick embryos was devised.

Materials & Methods:

A trial and error method using normal and daily laboratory glassware and naturally occurring nutrient rich food was performed. Chick embryos were grown in various containers through which developing embryo could be studied without having to break open through the container. The nutrients required for the development of the embryo was provided by daily and cheap food sources like milk, egg. The time period of the experiment allowed six trials to be performed which brought about partial success to the above objective of the experiment.

Results and Conclusion:

A protein rich combination of Milk whey, Albumen, Pannett-Compton saline where whey was higher in proportion was indicative of supporting the growing chick embryo and an improved culture vessel was designed which helped maintain the pH and also drain away the excess medium. It also helped restrict the position of the embryo in the vessel.