Research Article
Open Access
The effect of the antioxidant drug “U-74389G” on white blood cells levels during hypoxia reoxygenation injury in rats.
C. Tsompos’, C. Panoulis”, K Toutouzas’, G. Zografos‘, A. Papalois°
‘Constantinos Tsompos: consultant A, Department of Obstetrics & Gynecology, Mesologi
County Hospital, Etoloakarnania, Greece
Constantinos Panoulis: Assistant Professor, Department of Obstetrics & Gynecology,
Aretaieion Hospital, Athens University, Attiki, Greece
*Konstantinos Toutouzas: Assistant Professor, Department of Surgery, Ippokrateion
General Hospital, Athens University, Attiki, Greece
‘George Zografos: Professor, Department of Surgery, Ippokrateion General Hospital,
Athens University, Attiki, Greece
°Apostolos Papalois: Director, Exprerimental Research Center ELPEN Pharmaceuticals,
S.A. Inc., Co.
C. Tsompos, C. Panoulis, K Toutouzas, G. Zografos, A. Papalois. The effect of the antioxidant drug “U-74389G” on white blood cells levels during hypoxia reoxygenation injury in rats. Asian Journal of Pharmacology and Toxicology, 04(13), 2016, 22-32.
Abstract
Aim: The aim of this experimental study was to examine the effect of U-
74389G on rat model and particularly in a hypoxia - reoxygenation (HR)
protocol. The effect of that molecule was studied hematologically using
blood mean white blood cells levels.
Materials and methods: 40 rats of mean weight 231.875 g were used in the
study. White blood cells levels were measured at 60 min (groups A and C)
and at 120 min (groups B and D) of reoxygenation. U-74389G was
administered only in groups C and D.
Results: Results were that U-74389G administration kept significantly
increased the white blood cells level by 30.12%+10.87% (p=0.0050).
Reoxygenation time also kept significantly increased the white blood cells
level by 25.18%+11.64% (p=0.0300). However, U-74389G administration
and reoxygenation time together kept significantly increased the white
blood cells level by 23.64%+6.32% (p=0.0003).
Conclusions: Results of this study show that U-74389G administration,
reoxygenation time and their interaction kept short-term significantly
increased the white blood cells levels. The U-74389G administration which
has antioxidant capacity through oxygen free radical scavenging, could not
disrupt short-term the vicious cycle of HR and leukocytosis.
Keywords
hypoxia; reoxygenation; U-74389G; white blood cells