Anti-snake venom activities of ethanol and aqueous extract of Asparagus racernosus against Indian cobra (Naja naja) venom induced toxicity
Background: Snake bite is an occupational hazard that leads to significant number of fatalities and medical emergencies in developing countries. The focus of research in the field of snake bite management is now on the plant kingdom as across countries from time immemorial traditional healers have been using herbal drugs for management of snake bite.
Objective: To evaluate anti-snake venom activities of ethanol and aqueous extracts of Asparagus racernosus (AR) against Indian cobra (Naja naja) venom induced toxicity.
Materials and Methods: Anti-snake venom activities of Asparagus racernosus against Indian cobra (Naja naja) venom induced toxicity was studied via in vitro phospholipase A2 induced hemolysis on sheep RBC and Naja naja venom induced anticoagulation, in- vivo neutralization activity against lethality induced by Naja naja venom in rats, Naja naja venom induced edema and effect on bleeding time of venomized rats.
Results: The minimum lethal dose (MLD) was found as 2mglkg and both ethanol and aqueous extracts of Asparagus racernosus possess significant neutralization activity against Naja naja venom induced lethality. Both AR extracts inhibited phospholipase A2 dependent hemolysis of sheep RBC and exhibited significant reduction (p<0.001) in the anti-coagulant activity of venom with 71.87% and 78.87% inhibition by ethanol and aqueous extract of Asparagus racernosus (EAR) at 300 pg respectively. EAR and AAR at doses of 300, 600 and 900 mglkg exhibited significant (p<0.001) decrease in bleeding time and both extracts also displayed anti-inflammatory activity by inhibiting edema significantly (p<0.001).
Conclusion: These findings suggest a potential antivenom role of Asparagus racernosus against Naja naja envenomation.
Sunil Prashar. et al.: Asian Journal of Pharmacology and Toxicology, 04(16), 2016,01-08.